WP4 evaluates effects of river restoration by analysing existing data as well as performing field studies on paired catchments. The field studies will use examples of restored sites in which either one large scale measure (flagship restoration site) or smaller restoration measures (small restoration site) have been implemented. These restoration sites will be compared to “control sites” that are situated upstream and are still degraded (nested design). Deliverable 4.1. documents the abiotic and biotic parameters to be recorded at the case study sites and provides a description of the methods for field investigations in 2012 and 2013 including manuals, field forms and protocols.